ImageJ 1.43 n may therefore provide a useful tool for the assessment and discrimination of apoptotic cells. Consequently, a quantitative measure of apoptosis can be obtained that is linked to morphological changes.
CIRCULARITY IMAGEJ FREE
Nuclear area factor can be calculated using powerful free and open-source software. NAF was the most reliable parameter in assessment of apoptosis. Image analysis results correlated positively and significantly with the results obtained by cell viability assay (R = 0.958, P-value = 0.042). This decrease was assumed to indicate DNA loss. Some of the other studied parameters showed also the same trend. Image analysis revealed a progressive and highly significant decrease in nuclear area factor detected in apoptotic cells and in cells suspected of undergoing apoptosis compared to the control cells (P-values < 0.01). Cell viability assay and nuclear image analysis of stained Hep2 cells were used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells based on parameters such as nuclear area, circularity, perimeter and nuclear area factor (NAF), in association with visual morphology. Study DesignĬisplatin in different concentrations was used to induce apoptosis in cultured Hep2 cells. To verify the efficacy of ImageJ 1.43 n in determining the extent of apoptosis which is a complex and multistep process.
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